INSERTseq
Results

Summary

Peaks

Sample	Number of mapped reads	Number of initial peaks	Number of significant peaks	Number of unanchored peaks
201212_2_subset	48	2	1	15
201212_subset	48	2	1	15

All insertions

Sample	Chromosome	Start	End	Genome Browser
201212_2_subset	chr12	24551636	24552559
201212_subset	chr12	24551636	24552559

Sample 201212_2_subset

Summary of integrations

Initial quality check

Sample	201212_2_subset
Mean read length	621.6
Read length STDEV	680.7
Mean read quality	10.6
Number of initial reads	37,000.0
Total sequenced bases	999,857.0

Filtering and Trimming

Sample	201212_2_subset
Initial number of reads	37,000.0
Quality filtered reads	24426
Number of extracted UMIs	5625
Number of consensus UMI sequences	4665
Number of filtered and trimmed reads	3911

Sample 201212_subset

Summary of integrations

Initial quality check

Sample	201212_subset
Mean read length	621.6
Read length STDEV	680.7
Mean read quality	10.6
Number of initial reads	37,000.0
Total sequenced bases	999,857.0

Filtering and Trimming

Sample	201212_subset
Initial number of reads	37,000.0
Quality filtered reads	24426
Number of extracted UMIs	5625
Number of consensus UMI sequences	4665
Number of filtered and trimmed reads	3911

Run Parameters

Sample	adapter_1F	adapter_2	ontarget
201212_2_subset	AATGATACGGCGACCACCGAGATCTACACGAGTCTTGTGTCCCAGTTACCAGG	ACACTCTTCACGCTCTTCCGATC	GTAACTAGAGATCCCTCAGACCCTTT
201212_subset	AATGATACGGCGACCACCGAGATCTACACGAGTCTTGTGTCCCAGTTACCAGG	ACACTCTTCACGCTCTTCCGATC	GTAACTAGAGATCCCTCAGACCCTTT

UMI clustering	true
UMI type	TV
minimum UMI length	27
maximum UMI length	29
UMI clustering identity	0.99
UBS threshold	1
Adapter trimming error	0.2
Reference genome	hg38_chr12.softmasked.fasta
Output directory	Output

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