CRISPR-A helps
you from design
to analysis.

Simulate gene editing outcomes in your target.

Assess the quality of gene editing experiments using next generation sequencing (NGS) data.

How should we analyse your reads?

Type of Analysis: If you have two fastq files named R1 and R2, choose pair-end reads

Single-end Reads

Paired-end Reads

Control noise adding a negative control Optional

Drop your R1 files Press ctrl while selecting your files to upload several at the same time

Drop your R2 files Once uploaded, check that the each pair of files are printed in the same position. Visit Help for more info.

Analyse NGS datasets

Upload forward read:

Upload reverse read (optional):

Reference genome:

Human

Mouse

Other

Protospacer sequence:

Target sequence (optional):

Cut site location in protospacer:

Does this sample has a negative control sample? Use the keyboard arrows to move arround the mocks. You can select more than one by pressing "ctrl" and clicking

Do you need a highly precise analysis?

Uni-molecular barcodes:

Yes

Spikes:

Yes

Example results

Analyse simulated dataset

Cut site location: